fig6

Figure 6. Anticancer effect of agarose/Fe₃O₄ hydrogels. Live/dead staining of MDA-MB-231-Luc cells cultured with the agarose/Fe₃O₄ hydrogel discs via sitting (A), transwell (C), and adhesion (E) modes before and after AMF irradiation (live cell: green fluorescence, dead cells: red fluorescence). Quantified cell viability during culture without or with agarose/Fe₃O₄ hydrogel discs via sitting (B), transwell (D), and adhesion (F) modes before and after AMF irradiation. Cell viability was normalized to that cultured with agarose hydrogel without free Fe₃O₄ NPs before AMF irradiation. Data are the mean ± SD (n = 3). Significant difference: *P < 0.05, ***P < 0.001. N.S. : no significant difference.