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Figure 6. The regulation of neurogenesis by hieratical structures. (A) Schematic diagram of the fabrication of neural conduits with a hieratical structure. (B) Optical photograph of hollow conduits. (C) The photo of conduits filled with aligned hydrogel. (D) SEM images of conduits filled with silk nanofiber fillers. Laser scanning confocal microscopic images (E) and proliferation (F) of Schwann cells cultured on different concentrations of layered conduits for one, three, and seven days. (G) The distribution of orientation angle of Schwann cell nucleus after seven days. (H) ELISA for brain-derived neurotrophic factor (BDNF) secreted by cells on hieratical conduits. (I) Longitudinal and transverse H&E staining of regenerated nerves in vivo. (J) S-100 and NF-200 staining of regenerated nerves at
