fig9

Figure 9. Hierarchical bilayer structured scaffold for periodontal tissue regeneration. (A) The schematic diagram of manufacturing of hierarchical bilayer architectures. (B) SEM and confocal images of upper and lower layers from the top to the bottom. (C) Parallel-arranged CGF (P-CGF), randomly arranged CGF (R-CGF), and cell arrangement in the intrafibrillarly mineralized collagen (IMC) layer at day 1. (D) Micro-CT, H&E, and Masson trichrome staining of the periodontal tissue regeneration area of different groups. (E) Immunohistochemical staining of CD146, STRO-1, BMP2, COL-1, and VEGFR-1. Arrow: Positive cells. (F) Semiquantitative analysis of positive cell count of (E). Reproduced with permission^[111]. Copyright 2021, Publishing services by Elsevier BV on behalf of KeAi Communications Co. Ltd. *P < 0.05.